Our results probably present new insight into the regulation of six genes (PELI1, TIAM1, KRIT1, RBPJ, ZNF367, CPEB3) involved in the Notch signaling pathway by hsa-miR-21, which are related to patients with the Child–Pugh score C. Hence, a key mechanism and a potential target pathway in regulating liver disease is the Notch signaling pathway. Here, PELI1 is linked to liver disorder.