In the present study, we clarified the regulatory pathway of miR‐196a‐5p/NR6A1/E‐cadherin in inhibiting the proliferation, metastasis and neurogenesis of testicular tumor cell in vivo and in vitro, and demonstrated miR‐196a‐5p inhibits testicular tumor progression. The gene discussed is NR6A1; the disease is neoplasm of testis.