In the BCC dataset, the Mac_s2 macrophage subcluster overexpressed the TREM2hi macrophage marker genes (TREM2, FOLR2, MMP12, C1QA, C1QB, and C1Qc; Supplementary Fig. 9d); the B_sc2 B-cell subcluster overexpressed the B_c22 B cells marker genes (TRAC, IL2RA, ITGB1, ZBTB32, TRAF1, and CCND2; Supplementary Fig. 9e). The gene discussed is TREM2; the disease is skin basal cell carcinoma.