Pathway analysis revealed a significant reduction in ligand-receptor binding capacity, IFNα and IFNβ signaling, IFN-γ response, and immunoregulatory interactions of Tgd_c21 cells, suggesting that Tgd_c21 cells may be a type of ‘exhausted’ γδ T cell with impaired anti-tumor immune functions. The gene discussed is IFNG; the disease is neoplasm.