Furthermore, using HEK-293-TLR5 cells we observed TLR5 signalling 2 and 4 hours post infection with P. aeruginosa (p<0.01 and p<0.001) and also after P. histicola infection (p<0.001 and p0.01) with a significant reduction at 4 hours compared to 2 hours (p<0.01, Fig 2C). The gene discussed is TLR5; the disease is infection.