The basic assay used for analysis of cytotoxic Aβ and tau involves incubating endothelial cells with an infectious strain of P. aeruginosa in Hanks Balanced Salts Solution (HBSS), collecting the supernatant 4–5 h after infection, filter sterilizing to remove bacteria, adding the sterile supernatant to confluent cultures of naïve PMVECs and then assessing cell killing 21–24 h later (Fig. 1). Here, MAPT is linked to infection.