Although the interrelationship among EA, autophagy, and depression is interesting and blank, our data preliminarily provided the evidence that the occurrence of CUMS-induced depression-like behavior may be concerned with autophagy, and EA demonstrated antidepressant effects by partly inhibiting autophagy with the decreased level of LC3 and number of autolysosomes. This evidence concerns the gene MAP1LC3A and depressive symptom measurement.