Firstly, by two-colour super resolution-stimulated emission depletion (STED) microscopy we showed that SLC38A2 was located predominantly in the Trans–Golgi network (TGN) in MCF7 (Fig. S2A), consistent with previous analysis in normal and cancer cells.22 SLC38A2 localisation appeared typical of intracellular vesicles, present in proximity to the TGN and also other cytoplasmic regions (Fig. S2A). This evidence concerns the gene SLC38A2 and cancer.