We propose the levels of CXCL9 and CXCL10, the frequencies of CD4+CD25+ T-cells and the frequency of T. gondii-specific IFN-γ producing CD4+ T-cells presented as biomarkers able to distinguish with high accuracy infants with congenital toxoplasmosis from uninfected healthy controls. Here, CXCL10 is linked to congenital toxoplasmosis.