AKT1 and non-small cell lung carcinoma: To interrogate whether aaptamine-induced deactivation of PI3K/AKT/GSK3β axis disrupted the cell cycle progression of NSCLC cells, western blot assay of A549 and H1299 cells treated with aaptamine (0, 8, 16 and 32 μg/mL), respectively, for 48 h illuminated that aaptamine specifically decreased the ratios of p-PI3K/PI3K, p-AKT/AKT and p-GSK3β/GSK3β in a dose-dependent manner, which correspondingly downregulated their downstream cell cycle-specific driver kinases and proteins in NSCLC cells (Figure 5).