Based on the rationale that colon cancer stem cells (CSCs) are characterized by a predominant glycolytic metabolism [33, 34] and that TRAP1 is upregulated in colon CSCs and involved in their maintenance [11], patient‐derived CRC spheres, known to be enriched in CSCs [35], were evaluated to further establish the relationship between TRAP1, PFK1 activity/expression, and lactate production. Here, TRAP1 is linked to malignant colon neoplasm.