Since HCT116 and HT29 cells are routinely cultured in the presence of high glucose (16.6 mm), to rule out the hypothesis that the glycolytic profile of CRC cell lines with high TRAP1 expression may depend on glucose concentration, metabolic parameters were measured in HCT116 cells cultured in more physiologic glucose concentration (5.5 mm) upon TRAP1 silencing. The gene discussed is TRAP1; the disease is colorectal carcinoma.