Our results showed that (a) PRKAR2B enhances PCa cell glycolysis, (b) PRKAR2B is able to upregulate HIF‐1α protein level, (c) HIF‐1α transcriptionally induces PRKAR2B expression in PCa; (d) PRKAR2B‐mediated tumour growth in PCa cells is glycolysis‐dependent. Here, PRKAR2B is linked to neoplasm.