Here, we discovered that while CDDP showed anti-tumor effects via DNA damages and apoptosis, also activated 14-3-3ε/PI-3K/Akt signaling pathway and forming a survival mechanism, while high-dose cisplatin could not activate the above protective mechanism even result in severe adverse effects, which might be related to the antioxidant system. The gene discussed is AKT1; the disease is neoplasm.