We further independently validated TS-mediated EMT by knocking down TS in NSCLC cell lines SK-MES-1 (SCC cell line) and NCI-H23 (ADC cell line), where TS depletion led to upregulation of E-CAD and downregulation of VIM in SK-MES-1 (Supplementary Fig. 2H) and downregulation of VIM and ZEB1 (mesenchymal marker) in NCI-H23 (Supplementary Fig. 2I). This evidence concerns the gene TYMS and non-small cell lung carcinoma.