To determine the sequence of secretion of anti-ML-ESP Igs occuring during the enteral phase of infection with T. spiralis, the presence of IgE, IgA, IgG1 and IgG2a anti-ML-ESP secretory cells in Peyer’s patches, mesenteric lymph nodes and lamina propria cell suspensions from infected and non-infected rats were studied using ELISPOT. The gene discussed is CUBN; the disease is infection.