Interestingly, unlike the case with EVs, trastuzumab did not upregulate all of the indicated proteins in the breast cancer cells themselves: the drug upregulated RAB10 and PERP in BT-474 cells (Fig. 6d, e) but downregulated ITB1 (Fig. 6a) and did not change the levels of GNAS2 and GNA13 in the cells (Fig. 6b, c). This evidence concerns the gene RAB10 and breast cancer.