To assess the function of TIMP-2 in cancer cell lines, the expression of TIMP-2 was knocked down in ovarian cancer cell lines JOSH2 and OVCAR4 and FT282 Fallopian cell line using three unique 27mer siRNA duplexes individually, or a pooled siRNA duplex directed against human TIMP-2 (T2-KD). This evidence concerns the gene TIMP2 and ovarian carcinoma.