NAV1 and neuroblastoma: Given the limited material available, a single round of activity-guided fractionation using a high-throughput fluorescence assay assessing inhibition of veratridine-induced responses in the neuroblastoma cell line SH-SY5Y, expressing subtypes NaV1.2, 1.3, and 1.7 [32], identified compound(s) eluting in the buffer front on a Vydac 218TP C18 column as the bioactive components (Figure 1a).