However, in the current study, delivery of INS-FUR alone (AAV8-INS-FUR-mCherry) or INS-FUR together with Pdx1 (AAV8-INS-FUR-venus + AAV8-Pdx1), using the non-integrating AAV8 vector did not reverse hyperglycaemia and there was no evidence of expression of β-cell transcription factors that lead to pancreatic transdifferentiation. This evidence concerns the gene PDX1 and Hyperglycemia.