When a logistic regression model was adjusted for female gender, atrial fibrillation, maximum axillary temperature, NIHSS at admission, volume of ischemic lesion, effective reperfusion, symptomatic HT or LA, and each biomarker separately, glutamate (OR: 1.01; CI 95%: 1.00–1.01, P < 0.0001), TNF‐α (OR: 1.05; CI 95%: 1.01–1.09, P = 0.009), NSE (OR: 2.13; CI 95%: 1.50–2.90, P < 0.0001), and S100‐β (OR: 5.86; CI 95%: 7.05–56.12, P < 0.0001) were independently associated with poor outcome at 3 months in patients with IS through the connection with LA presence. This evidence concerns the gene S100B and atrial fibrillation.