In the A549 lung carcinoma/HepG2 HCC cell model for AhR gene reporter plasmid expression [64], applied TGFβ1 results in activation of z, x-plane transcription-ready plasmid upon endogenous TGIF1 transcription at Peff 0.080 as the SMAD co-adapter is required for RNA polymerase transcription, however without the need for applied TGFβ1 in A549 cells that demonstrate a bidirectional negative expansion Δ Cmicro response Peff of 0.080 as compared the nadir for HepG2 cells, which would between Peff 0.130 (LGAL1) and 0.106 (UGT1A1). The gene discussed is TGFB1; the disease is hepatocellular carcinoma.