To explore the effects of Cdc6 on the apoptosis of PC cells, we performed staining analysis using annexin V-fluorescein isothiocyanate (FITC), which recognizes phospholipid phosphatidylserine on the outer membrane of apoptotic cells, and propidium iodide (PI), a marker of cell membrane permeability26. Here, ANXA5 is linked to pachyonychia congenita.