CDC6 and pachyonychia congenita: Next, we analyzed the expression of Cdc6 in PC cell lines by western blot analysis, and observed that Cdc6 was up-regulated in various PC cell lines including AsPC-1, PANC-1, MIA PaCa-2, and Capan-1 cells compared to human umbilical vein endothelial cells (HUVEC) and normal human pancreatic duct epithelial (HPDE) cells (Fig. 1C).