Since we were particularly interested in the capacity of IL-33 to recruit monocytes, which differentiate into macrophages within the brain, we ordered monocytes, differentiating BMDM (BMDM 3), and glioma-associated BMDM (BMDM 2) within the IL-33+ xenograft in a pseudo-time trajectory to understand this transition (Fig. 10g). The gene discussed is IL33; the disease is glioma.