TLR4 in trophoblasts plays a key role in the development of inflammatory metabolism in the placenta.[21] We also detected the TLR4/MyD88/NF-κB pathway in the placentas because TLR4/MyD88/NF-κB with insulin resistance in placentas of GDM act as a key regulatory factor.[40, 41]. This evidence concerns the gene NFKB1 and gestational diabetes.