Notably, in non–MYCN-amplified NB (SH-EP, SH-SY5Y, and SK-N-AS cells), CYC065 still potently reduced RNAPII Ser2P at compound concentrations that had no or little impact on apoptosis (Supplemental Figure 2, I and J), suggesting that transcriptional inhibition is not lethal in these non–MYCN-amplified cells. The gene discussed is MYCN; the disease is neuroblastoma.