TFR2 and lysosomal storage disease: LC3 is present on autophagosomal membranes and phagocytic membranes during LC3-assisted phagocytosis.18,22 TFR2 is endocytosed during transferrin uptake and either recycled back to the plasma membrane through recycling endosomes or delivered via multivesicular bodies to the lysosome for degradation.7 Intracellular accumulation of vacuoles is a histopathological finding indicative of lysosomal storage diseases, where the vacuoles have been interpreted as secondary lysosomes containing partially or undegraded substrates.