To explore whether 8-oxo-dG mediated the effect of mtDNAcn on NAFLD, a mediation analysis was conducted after adjusting for gender, age, BMI, WC, HDL-C, LDL-C, TG, UA, glucose tolerance status, HOMA-IR, blood pressure, ALT, AST, and IL-6. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.