Interestingly, NHBE3 cells, which were derived from a healthy donor with no smoking history and no cardiovascular disease, showed the highest average percentages of FOXJ1+ cells and the lowest average percentages of MUC5AC + cells among all six groups of HBE cells in the ALI cultures under normoxia, intermittent H/R and 9-day consecutive hypoxia (green labels in Supplementary Figures S1A,B). The gene discussed is MUC5AC; the disease is cardiovascular disorder.