APP and Alzheimer disease: We further confirmed the mechanisms responsible for the effect of Prickle2 on the etiology of AD by transfection with an empty vector (Vec), pcDNA3/prickle2, si-NC and si-Prickle2 to a cell model of AD (N2a/APP695sw) by stably expressed human APP-695 Swedish mutation (K595N/M596L) in N2a cells.