By manipulating miR-146a-5p or/and TRAF6 levels in NSCLC cell lines, we found that (1) Overexpression of miR-146a-5p resulted in cell proliferation and reduced TRAF6 expression; (2) Inhibition of miR-146a-5p decreased cancerous cellular behavior, accompanied by higher TRAF6 levels; (3) TRAF6 gene silencing by siRNA recapitulate the outcome of miR-146a-5p over-expression; (4) Forced expression of TRAF6 in conjunction with miR-146a-5p overexpression sufficed to revoke the oncogenic effects of miR-146a-5p. Here, TRAF6 is linked to non-small cell lung carcinoma.