Thus, to overcome the potential effect of IWR-1-endo on unrelated host cell functions and establish a direct link between infection and Wnt/β-catenin activation, we infected naïve or IFNγ-stimulated BMDMs with a mutant Toxoplasma strain (RHΔASP5) and quantified parasite burden using qPCR. The gene discussed is IFNG; the disease is infection.