We additionally set up a rescue model and found no marked changes in glioma cell proliferation, migration, and invasion upon cotransfection of miR-29a-5p inhibitor and si-DHRS4, compared with si-Ctrl treatment alone, thus indicating that DHRS4 promotes the proliferation, invasion, and migration of glioma cells and is closely associated with the expression of miR-29a-5p. Here, DHRS4 is linked to central nervous system cancer.