The result showed that AFP-negative HCC patients had a lower incidence of distant metastasis (P < 0.001, χ2 test), intrahepatic metastasis (P = 0.007, χ2 test), and portal vein invasion (P = 0.016, χ2 test) compared with AFP-positive HCC patients. The gene discussed is AFP; the disease is hepatocellular carcinoma.