In this study, we found that 10 sphingolipids (2 Cer, 1 C1P, 2 HexCer, 1 dhSph, 1 dhS1P, 1 dhC1P, 1 HexSph, 1 S1P) including Sphingosine (d18:1)-1-P exhibit significant difference between HCC or AFP-negative HCC and cirrhosis. Here, AFP is linked to hepatocellular carcinoma.