CD274 and neoplasm: Since the traditional biomarkers for immunotherapy, such as the PD-L1 expression and tumor mutation burden (TMB) appeared to have limited predicting values in several clinical trials using combined immunotherapy (11, 12, 22, 24, 25), we investigated the values of a few new biomarkers in predicting and monitoring the therapeutic response and prognosis in second-line or higher settings when ICI was combined with anti-angiogenic therapy.