The KEGG pathway analysis indicated that RNA transport, ubiquitin mediated proteolysis, TNF signaling pathway, amino sugar and nucleotide sugar metabolism, insulin signaling pathway, p53 signaling pathway, base excision repair, and cell cycle, etc. were the most prominent pathways enriched in proteins with altered ubiquitination, which are associated with tumor metastasis. The gene discussed is TNF; the disease is neoplasm.