Therefore, we first analyzed the steady state levels of Tn antigen on the mouse CRC cell line MC38 (MC38 wild type, MC38-WT), using the GalNAc-specific snail lectin Helix Pomatia agglutinin (HPA), and observed that our MC38-WT cells displayed only intermediate Tn antigen levels (Figure 1A). Here, HPSE is linked to colorectal carcinoma.