After confirming that depleted LINC00673 was able to up-regulate CDKN2C and promote the cell cycle, we conducted a ChIP assay using antibodies against H3K27me3 in EC9706 and KYSE510 cells and found that H3K27me3 was enriched at the promoter region of CDKN2C. Some key molecules that may regulate CDKN2C expression via methylation modification—DNMT1, DNMT3A, and EZH2 (36–38)—were knocked down in ESCC cells; among these molecules, only EZH2 knockdown could up-regulate CDKN2C. Here, CDKN2C is linked to esophageal squamous cell carcinoma.