Activation of CD4+ T cells could promote infiltration of other detected immune subsets (e.g., CD8+ T cells and macrophages), while depletion of CD4+ T cells (but not CD8+ T cells and monocytes) abrogated immune infiltration, inflammation and fibrosis, which not only confirms our hypothesis but is consistent with previous publications about the role of CD4+ T cells in NAFLD progression (11, 27, 28). Here, CD8A is linked to metabolic dysfunction-associated steatotic liver disease.