Consistent with the previously published findings (16), we confirm that employing an intravenous route for both parasite and huRBC delivery, clodronate-loaded liposomal suspension for macrophage suppression, and the IL-2R-γ mutation on NOD/SCID genetic background, helped in achieving a model with greater reproducibility which supported the infection with different strains, including the severely attenuated C9-M. Here, IL2RG is linked to infection.