As shown in Figures 8A, B, there is a clear dose-dependent inhibition of expression of both phosphorylated forms of Akt and phosphorylated 4EBP1 when they are normalized to total Akt or 4EBP1 protein, indicating inhibition of PI3K/mTOR important pathway in U87 MG GBM tumor tissue from mice administered PBI-05204. This evidence concerns the gene EIF4EBP1 and glioblastoma.