Next, we examined if PBI-05204 affects the expression of stem cell markers including SOX2, a well-known stem cell transcription factor in cancer stem cells including GSCs (Auffinger et al., 2014; Bulstrode et al., 2017; Guerra-Rebollo et al., 2019; Penaranda-Fajardo et al., 2019). This evidence concerns the gene SOX2 and cancer.