To determine whether the increased DNA methylation could account for decreased expression of Arc, Bdnf and Reln gene after sevoflurane, we firstly treated C6 glioma cells with pharmacological inhibitor 5-Aza-2′-deoxycytidine (5-Aza, 10 μM in cell culture) 60 min before sevoflurane anesthesia. Here, BDNF is linked to central nervous system cancer.