In C6 glioma cells, the mRNA levels of Dnmt1 (141.00 ± 26.30 vs. 100.00 ± 21.41, P = 0.0142, Figure 4E), Dnmt3a (132.20 ± 23.42 vs. 100.00 ± 25.62, P = 0.0464, Figure 4F) and Mecp2 (140.30 ± 31.41 vs. 100.00 ± 30.37, P = 0.0472, Figure 4G) increased and Dnmt3b mRNA levels decreased (100.00 ± 16.62, 77.72 ± 17.15, P = 0.0453, Figure 4H) after sevoflurane anesthesia. The gene discussed is MECP2; the disease is central nervous system cancer.