Moreover, TLR4 siRNA transfection alleviated PA-stimulated increases in the CD11c and CD206 expression, JNK/AP1 and IKKβ/NF-κB pathway activation, and changes of inflammatory factor levels in supernatants and herein ameliorated PA-stimulated insulin resistance and lipid deposition in cocultured hepatocytes. The gene discussed is NFKB1; the disease is Insulin resistance.