The most frequently affected genes were those previously reported in WT in multiple studies—CTNNB1, DROSHA, WT1, DGCR8, XPO5, AMER1, DICER1 and TRIM28. Mutations in MAX, MLLT1, MYCN, KRAS, SIX1 and SIX2 were each identified in a single tumour (Fig. 3b). This evidence concerns the gene AMER1 and neoplasm.