In this study, we performed high-throughput RNA-sequencing (RNA-Seq) in four paired Huh7 hepatoma cell cultures continuously treated with 10 ng/ml TGF-β or DMSO for 7 days to comprehensively identify differentially expressed lncRNAs, circRNAs, miRNAs, and mRNAs. The gene discussed is TGFB1; the disease is hepatocellular carcinoma.