CD276 and colorectal carcinoma: RT-qPCR and Western blot further confirmed that B7-H3 expression was significantly increased in stable B7-H3-overexpressing CRC cell lines (Supplementary Fig. S1b, c), while the B7-H3 level was obviously decreased in stable B7-H3-knockdown CRC cell lines (Supplementary Fig. S1d, e).