To validate the predictive power of LCP1 and ADPGK, we further collected a validation cohort of 28 cancer patients receiving anti-PD-1/PD-L1 inhibitors with both high-quality formalin-fixed paraffin-embedded (FFPE) pre-treatment tumor tissues and clinicopathological information (Supplementary Table 9 and Supplementary Fig. 11). This evidence concerns the gene CD274 and cancer.