The frequency of CD80+CD86+ mature BMDCs after co-culture with 4T1 tumour cells pretreated with free OLE was significantly higher that of the control group, indicating that ICD from dying 4T1 cells elicited by OLE can induce DC maturation and immune response (Fig. 4a, b, and Supplementary Fig. 22). This evidence concerns the gene CD86 and neoplasm.