However, this is not the case since: 1) pharmacological blocker and genetic manipulation of TRPV1 could alter [Ca2+]i in GC cells, 2) genetic manipulation of TRPV1 could alter GC cell proliferation, migration and invasion both in vitro and in vivo, and 3) [Ca2+]i chelator BAPTA-AM efficiently prevented TRPV1-mediated CaMKKβ activation and AMPK phosphorylation in GC cells. The gene discussed is TRPV1; the disease is gastric cancer.