We then quantified the expression of related proteins in tumor tissues and found that upon knockdown of UbcH10 and KIAA0101, the expression of the SAC components and cell cycle-associated proteins BubR1, Mad2 and CyclinB was effectively restored in tumor tissues (p<0.05 compared to the control group), and the co-silenced group showed a superior effect on these proteins (p<0.01 compared to the model group) (Fig. 6b). The gene discussed is PCLAF; the disease is neoplasm.