In addition, M2-mAchR/Src signaling was found to be involved in DCA induced TLR2 tyrosine phosphorylation, an important parallel phenomena was described for EGFR, which is also transactivated by bile acids in a muscarinic receptor-dependent or TGR5-mediated manner and contributes to the cancer cell proliferation and survival,24,30,40 thus crosstalk between bile acid receptor and other co-expressed receptors may represent an important fashion that bile acids play their diverse roles, and the regulation of intermediate process should be further elucidated. This evidence concerns the gene TLR2 and cancer.