In order to reduce the effect of genetic variability on the study of this pathology, a characterization of hiPSC lines derived from fibroblasts of the PD affected monozygotic twin, unaffected twin, and a subject with sporadic PD, and healthy subjects have been established; markers for three-germ layer differentiation APOE and CTNNB1 (Endoderm), ITGB1 and CDH1 (Mesoderm), and FGFR2 and CRABP2 (Ectoderm) have been evaluated using the NanoString approach [55]. The gene discussed is CDH1; the disease is Parkinson disease.